This chapter provides an overview of the regulatory aspects of genetically modified organisms (GMOs). It also updates on the recent initiatives taken by leading statutory agencies on new developments in biotechnology. It is complemented with updated information. As the cultivation of GMOs enters into its landmark 25th year, the readers of this book will get a peek into the regulations governing GMOs from an Indian perspective. An overview of the approaches for GMO legislations applicable in the USA, EU, and India is presented to give the readers an idea of the similarities and differences between the GMO legislations of the three different jurisdictions.